147 locations (2025), -3.3% YoY(Item 20)
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Median Revenue (AUV)
$646,462
Source: 2025 FDD, Item 19
Initial Investment
$116,295 – $169,795
Source: 2025 FDD, Item 7
Franchise Fee
$60,000
Source: 2025 FDD, Item 5
Royalty
7.5%
Source: 2025 FDD, Item 6
About Bio-One
Bio-One is a restoration services franchise providing residential and commercial removal of regulated and non-regulated bio-medical waste. Additional services include cleaning, disinfecting, hoarding remediation, medical waste, and sharp-instruments removal, targeting both the general public and governmental institutions such as police and fire departments.
Gross Sales by Performance TierSource: 2025 FDD Item 19
AUV Trend — Multi-Year Median Revenue
Additional Item 19 Analysis
Deeper Item 19 Analysis (Charts 3–6)
Openings & Closings, Transfer Rates, Franchise vs Corporate Mix
Bio-One vs Competitors
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Per Item 7 of the 2025 FDD Franchise Disclosure Document, the estimated initial investment ranges from $116,295 to $169,795.
The FDD reports an initial franchise fee of $60,000.
The FDD reports an ongoing royalty of 7.5%.
The 2025 FDD reports a median revenue per unit of $646,462 for fiscal year 2024. Individual results vary.
Many franchise purchases are financed with SBA 7(a) loans covering a large share of the total project cost. You can check what you'd qualify for with Franchable's free SBA prequalification.
Data sourced from the brand's Franchise Disclosure Document. Franchable is not affiliated with or endorsed by Bio-One. This is not an offer to sell a franchise. Individual results vary.