Bio-One Franchise: Cost, Fees & Earnings
147 locations (2025), -3.3% YoY(Item 20)
A Bio-One franchise requires an estimated total investment of $134,645 to $221,095, including a $60,000 initial franchise fee (latest FDD, Item 7). Franchisees pay an ongoing royalty of 7.5% (Item 6). Bio-One's FDD includes an Item 19 financial performance representation, but Franchable does not currently publish a system-wide unit revenue figure from it.
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Initial Investment
$134,645 – $221,095
Source: FDD, Item 7
Franchise Fee
$60,000
Source: FDD, Item 5
Royalty
7.5%
Source: FDD, Item 6
Bio-One Franchise Key Facts
| Metric | Value | Source |
|---|---|---|
| Initial Investment | $134,645 – $221,095Source: FDD, Item 7 | |
| Initial Franchise Fee | $60,000Source: FDD, Item 5 | |
| Royalty | 7.5%Source: FDD, Item 6 | |
| Median Revenue (AUV) | Bio-One's FDD includes an Item 19 financial performance representation, but Franchable does not currently publish a system-wide unit revenue figure from it.Source: Item 19 | |
| Total Locations | 147 (2025)Source: Item 20 |
Bio-One Franchise Fees
Initial franchise fee(Item 5)
$60,000
Ongoing royalty(Item 6)
7.5%
National ad / brand fund(Item 6)
2%
Local marketing spend(Item 6)
5%
Technology fee(Item 6)
$7,800/mo
Buildout / construction(Item 7)
$0 – $1,500
Additional Item 5–6 fees (transfer, renewal, area development, and any veteran discounts) are not yet parsed on Franchable — see the brand's FDD for the full fee schedule.
About Bio-One
Bio-One is a restoration services franchise providing residential and commercial removal of regulated and non-regulated bio-medical waste. Additional services include cleaning, disinfecting, hoarding remediation, medical waste, and sharp-instruments removal, targeting both the general public and governmental institutions such as police and fire departments.
What Bio-One's FDD Discloses
The largest disclosed cost line in Bio-One's disclosed unit P&L was Payroll and Personnel, at about 35.2% of revenue (disclosed Item 19, FY2025).
Gross Sales by Performance TierSource: FDD Item 19
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Explore all brands →AUV Trend — Multi-Year Median Revenue
Additional Item 19 Analysis
Deeper Item 19 Analysis (Charts 3–6)
Openings & Closings, Transfer Rates, Franchise vs Corporate Mix
Bio-One vs Competitors
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Bio-One Franchise FAQ
How much does a Bio-One franchise cost?
Per Item 7 of the latest Franchise Disclosure Document, the estimated initial investment ranges from $134,645 to $221,095.
What is the Bio-One franchise fee?
The FDD reports an initial franchise fee of $60,000.
What royalty does Bio-One charge?
The FDD reports an ongoing royalty of 7.5%.
How much does a Bio-One franchise owner make?
Bio-One's FDD includes an Item 19 financial performance representation, but Franchable does not currently publish a system-wide unit revenue figure from it. Review Item 19 of the FDD and ask the franchisor for validation contacts before relying on any earnings estimate.
Can I finance a Bio-One franchise with an SBA loan?
Many franchise purchases are financed with SBA 7(a) loans covering a large share of the total project cost. You can check what you'd qualify for with Franchable's free SBA prequalification.
Data sourced from the brand's Franchise Disclosure Document. Franchable is not affiliated with or endorsed by Bio-One. This is not an offer to sell a franchise. Individual results vary.