Bio-One Franchise: Cost, Fees & Earnings
147 locations (2025), -3.3% YoY(Item 20)
A Bio-One franchise requires an estimated total investment of $116,295 to $169,795, including a $60,000 initial franchise fee (2025 FDD, Item 7). Franchisees pay an ongoing royalty of 7.5% (Item 6). The 2025 FDD reports a median unit revenue of $646,462 for FY2024 (Item 19).
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Median Revenue (AUV)
$646,462
Source: 2025 FDD, Item 19
Initial Investment
$116,295 – $169,795
Source: 2025 FDD, Item 7
Franchise Fee
$60,000
Source: 2025 FDD, Item 5
Royalty
7.5%
Source: 2025 FDD, Item 6
Bio-One Franchise Key Facts
| Metric | Value | Source |
|---|---|---|
| Initial Investment | $116,295 – $169,795Source: 2025 FDD, Item 7 | |
| Initial Franchise Fee | $60,000Source: 2025 FDD, Item 5 | |
| Royalty | 7.5%Source: 2025 FDD, Item 6 | |
| Median Revenue (AUV) | $646,462Source: 2025 FDD, Item 19 | |
| Total Locations | 147 (2025)Source: Item 20 |
Bio-One Franchise Fees
Initial franchise fee(Item 5)
$60,000
Ongoing royalty(Item 6)
7.5%
National ad / brand fund(Item 6)
2%
Local marketing spend(Item 6)
5%
Technology fee(Item 6)
$7,800
Buildout / construction(Item 7)
$0 – $1,500
Additional Item 5–6 fees (transfer, renewal, area development, and any veteran discounts) are not yet parsed on Franchable — see the brand's FDD for the full fee schedule.
About Bio-One
Bio-One is a restoration services franchise providing residential and commercial removal of regulated and non-regulated bio-medical waste. Additional services include cleaning, disinfecting, hoarding remediation, medical waste, and sharp-instruments removal, targeting both the general public and governmental institutions such as police and fire departments.
What Bio-One's FDD Discloses
The largest disclosed cost line in Bio-One's disclosed unit P&L was Payroll and Personnel, at about 35.2% of revenue (disclosed Item 19, FY2025).
Gross Sales by Performance TierSource: 2025 FDD Item 19
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Explore all brands →AUV Trend — Multi-Year Median Revenue
Additional Item 19 Analysis
Deeper Item 19 Analysis (Charts 3–6)
Openings & Closings, Transfer Rates, Franchise vs Corporate Mix
Bio-One vs Competitors
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Bio-One Franchise FAQ
How much does a Bio-One franchise cost?
Per Item 7 of the 2025 FDD Franchise Disclosure Document, the estimated initial investment ranges from $116,295 to $169,795.
What is the Bio-One franchise fee?
The FDD reports an initial franchise fee of $60,000.
What royalty does Bio-One charge?
The FDD reports an ongoing royalty of 7.5%.
How much revenue does a Bio-One franchise generate?
The 2025 FDD reports a median revenue per unit of $646,462 for fiscal year 2024. Individual results vary.
How much does a Bio-One franchise owner make?
Bio-One disclosed median unit revenue of $646,462 in FY2024 (Item 19). It does not disclose unit-level profit, so a reliable owner-income figure can't be drawn from its FDD. Individual results vary.
Can I finance a Bio-One franchise with an SBA loan?
Many franchise purchases are financed with SBA 7(a) loans covering a large share of the total project cost. You can check what you'd qualify for with Franchable's free SBA prequalification.
Data sourced from the brand's Franchise Disclosure Document. Franchable is not affiliated with or endorsed by Bio-One. This is not an offer to sell a franchise. Individual results vary.